Hotspot

psbT-pbf1

LSC · 76016-76087 · 72 bp

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Pi 0.0417
MarkerSeek score 65.30
Primer available yes
Label psbT-pbf1

Feature Metadata

label_name
psbT-pbf1
feature_type
igs
region
LSC
length_bp
72
variable_sites
3
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-pbf1_p1 TCGAGAACCGCCTAAAGTTC TTCGAGGAACACGGAGACTA 50.0 50.0 57.6 57.8 133 bp 1.0 77.4
psbT-pbf1_p2 TCGAGAACCGCCTAAAGTTC TCGAGGAACACGGAGACTAG 50.0 55.0 57.6 58.3 132 bp 1.0 76.8
psbT-pbf1_p3 GAGAACCGCCTAAAGTTCCA TTCGAGGAACACGGAGACTA 50.0 50.0 57.5 57.8 131 bp 1.0 76.6
psbT-pbf1_p4 GAGAACCGCCTAAAGTTCCA TCGAGGAACACGGAGACTAG 50.0 55.0 57.5 58.3 130 bp 1.0 76.0
psbT-pbf1_p5 TCGAGAACCGCCTAAAGTTC CATTCGAGGAACACGGAGAC 50.0 55.0 57.6 58.4 135 bp 1.0 75.9