Hotspot

petL-petG

LSC · 67703-67883 · 181 bp

Back to Ruta

Pi 0.0223
MarkerSeek score 63.90
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
181
variable_sites
4
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 TTTCCTTTAGCTCCGCCATG TGCAAATAGGCGGTTACGAA 50.0 45.0 58.2 57.9 375-377 bp 1.0 78.5
petL-petG_p2 TTTCCTTTAGCTCCGCCATG CAACTGATCACCACGTCTGT 50.0 50.0 58.2 57.8 397-399 bp 1.0 78.0
petL-petG_p3 TTTCCTTTAGCTCCGCCATG TCTGTACTGCAAATAGGCGG 50.0 50.0 58.2 57.7 382-384 bp 1.0 76.8
petL-petG_p4 TTTCCTTTAGCTCCGCCATG CTGCAAATAGGCGGTTACGA 50.0 50.0 58.2 58.4 376-378 bp 1.0 76.5
petL-petG_p5 TTTCCTTTAGCTCCGCCATG GATCACCACGTCTGTACTGC 50.0 55.0 58.2 58.4 392-394 bp 1.0 76.3