Hotspot

petA-psbJ

LSC · 65010-66084 · 1075 bp

Back to Ruta

Pi 0.0113
MarkerSeek score 66.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1075
variable_sites
12
pi
0.0113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.0 58.1 1217-1224 bp 1.0 81.1
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC GCTGGTATTCCTGTGATCGG 50.0 55.0 58.0 58.1 1269-1276 bp 1.0 80.5
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC TCCGGATTAGGTTCGTCCTT 50.0 50.0 58.0 58.1 1218-1225 bp 1.0 80.4
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.2 58.1 1214-1221 bp 1.0 80.0
petA-psbJ_p5 GCATCTGTTGTTTTGGCACA CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1182-1189 bp 1.0 79.7