Hotspot

ndhK-ndhC

LSC · 52232-52301 · 70 bp

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Pi 0.0351
MarkerSeek score 65.30
Primer available yes
Label ndhK-ndhC

Feature Metadata

label_name
ndhK-ndhC
feature_type
igs
region
LSC
length_bp
70
variable_sites
2
pi
0.0351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK-ndhC_p1 CCATACAGAAGCGGCCATAA GTTGGCTCAATTTACGCGTG 50.0 50.0 57.7 58.4 224-237 bp 1.0 79.5
ndhK-ndhC_p2 CCATACAGAAGCGGCCATAA AGTTGGCTCAATTTACGCGT 50.0 45.0 57.7 58.5 225-238 bp 1.0 78.8
ndhK-ndhC_p3 ACAGAAGCGGCCATAAACTG GTTGGCTCAATTTACGCGTG 50.0 50.0 58.5 58.4 220-233 bp 1.0 77.1
ndhK-ndhC_p4 ACAGAAGCGGCCATAAACTG AGTTGGCTCAATTTACGCGT 50.0 45.0 58.5 58.5 221-234 bp 1.0 76.5
ndhK-ndhC_p5 CCATACAGAAGCGGCCATAA TGGCGAAAAGGAGCATTAGA 50.0 45.0 57.7 57.2 206-219 bp 1.0 76.5