Hotspot

trnE-UUC-trnT-GGU

LSC · 32356-33204 · 849 bp

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Pi 0.0212
MarkerSeek score 70.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
849
variable_sites
18
pi
0.0212
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 936-953 bp 1.0 80.3
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 934-951 bp 1.0 80.3
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA AAACGCAGAAAAGCCCCTTA 50.0 45.0 57.9 58.0 964-981 bp 1.0 80.2
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA AAACGCAGAAAAGCCCCTTA 50.0 45.0 57.9 58.0 962-979 bp 1.0 80.2
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 990-1007 bp 1.0 80.1