Hotspot

trnD-GUC-trnY-GUA

LSC · 31669-32136 · 468 bp

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Pi 0.0171
MarkerSeek score 63.40
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
468
variable_sites
8
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 538-541 bp 1.0 75.5
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 537-540 bp 1.0 75.4
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 559-562 bp 1.0 74.5
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 558-561 bp 1.0 74.3
trnD-GUC-trnY-GUA_p5 TTTGATCTGTCGGGACTGAC TGGCAATATGTCTACGCTGG 50.0 50.0 57.3 57.8 592-595 bp 1.0 73.2