Hotspot

rpoB-trnC-GCA

LSC · 27437-28690 · 1254 bp

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Pi 0.0216
MarkerSeek score 74.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1254
variable_sites
26
pi
0.0216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCCGTATTTCCATCTCCGA CCACCAAGCCGAATAAGGAA 50.0 50.0 57.9 57.9 1347-1394 bp 1.0 82.7
rpoB-trnC-GCA_p2 TCCCGTATTTCCATCTCCGA GTTTGTTGATCAGGCGACAC 50.0 50.0 57.9 57.7 1313-1360 bp 1.0 81.3
rpoB-trnC-GCA_p3 TCCCGTATTTCCATCTCCGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1275-1322 bp 1.0 81.1
rpoB-trnC-GCA_p4 GGTATTGTCGACATTCCCGT CCACCAAGCCGAATAAGGAA 50.0 50.0 57.7 57.9 1361-1408 bp 1.0 80.9
rpoB-trnC-GCA_p5 TCCCGTATTTCCATCTCCGA GCGACACCCAGATTTGAACT 50.0 50.0 57.9 58.5 1300-1347 bp 1.0 80.1