Hotspot

trnR-UCU-atpA

LSC · 9987-10204 · 218 bp

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Pi 0.0193
MarkerSeek score 68.20
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
218
variable_sites
4
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AATGGTCAAAGGCGTCCATT GGTATTTACCGAGGAAGCCG 45.0 55.0 58.1 58.1 386-395 bp 1.0 81.4
trnR-UCU-atpA_p2 AATGGTCAAAGGCGTCCATT TCAGGAACAAAAGGAACGCT 45.0 45.0 58.1 57.7 344-353 bp 1.0 80.1
trnR-UCU-atpA_p3 AATGGTCAAAGGCGTCCATT ATTTACCGAGGAAGCCGAAG 45.0 50.0 58.1 57.7 383-392 bp 1.0 80.0
trnR-UCU-atpA_p4 GTCAAAGGCGTCCATTGTCT GGTATTTACCGAGGAAGCCG 50.0 55.0 58.5 58.1 382-391 bp 1.0 78.2
trnR-UCU-atpA_p5 AATGGTCAAAGGCGTCCATT AAGCCGAAGCCCTTTTGAAA 45.0 45.0 58.1 58.6 372-381 bp 1.0 77.8