Hotspot

trnS-GCU-trnG-UCC

LSC · 8075-8909 · 835 bp

Back to Ruta

Pi 0.0192
MarkerSeek score 58.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
835
variable_sites
16
pi
0.0192
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCGACGCTTTAATCCACTCA TGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.6 903-922 bp 1.0 54.2
trnS-GCU-trnG-UCC_p2 CCGACGCTTTAATCCACTCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 900-919 bp 1.0 53.3
trnS-GCU-trnG-UCC_p3 CCGACGCTTTAATCCACTCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 902-921 bp 1.0 52.2
trnS-GCU-trnG-UCC_p4 ACGCTTTAATCCACTCAGCC TGAACGAATCACACTTTTACCAC 50.0 39.1 58.3 57.6 900-919 bp 1.0 52.2
trnS-GCU-trnG-UCC_p5 CGCTTTAATCCACTCAGCCA TGAACGAATCACACTTTTACCAC 50.0 39.1 58.3 57.6 899-918 bp 1.0 52.2