Hotspot

rps12

IRa · 139179-139978 · 800 bp

Back to Ruscus

Pi 0.00125
MarkerSeek score 59.50
Primer available yes
Label rps12

Feature Metadata

label_name
rps12
feature_type
gene
region
IRa
length_bp
800
variable_sites
1
pi
0.00125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12_p1 TGACATGAGTGCGAAAGAGG ATTGAACGAGGAGCCGTATG 50.0 50.0 57.9 57.8 949 bp 1.0 77.6
rps12_p2 GACATGAGTGCGAAAGAGGT ATTGAACGAGGAGCCGTATG 50.0 50.0 57.9 57.8 948 bp 1.0 77.6
rps12_p3 CAGTACCTCGACGTGACATG ATTGAACGAGGAGCCGTATG 55.0 50.0 58.1 57.8 962 bp 1.0 77.5
rps12_p4 TGACATGAGTGCGAAAGAGG AAGAATTGAACGAGGAGCCG 50.0 50.0 57.9 58.3 953 bp 1.0 77.2
rps12_p5 GACATGAGTGCGAAAGAGGT AAGAATTGAACGAGGAGCCG 50.0 50.0 57.9 58.3 952 bp 1.0 77.2
rps12_p1 ATTGAACGAGGAGCCGTATG TGACATGAGTGCGAAAGAGG 50.0 50.0 57.8 57.9 949 bp 1.0 77.6
rps12_p2 ATTGAACGAGGAGCCGTATG GACATGAGTGCGAAAGAGGT 50.0 50.0 57.8 57.9 948 bp 1.0 77.6
rps12_p3 ATTGAACGAGGAGCCGTATG CAGTACCTCGACGTGACATG 50.0 55.0 57.8 58.1 962 bp 1.0 77.5
rps12_p4 AAGAATTGAACGAGGAGCCG TGACATGAGTGCGAAAGAGG 50.0 50.0 58.3 57.9 953 bp 1.0 77.2
rps12_p5 AAGAATTGAACGAGGAGCCG GACATGAGTGCGAAAGAGGT 50.0 50.0 58.3 57.9 952 bp 1.0 77.2