Hotspot

rpl23

IRa · 151512-151793 · 282 bp

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Pi 0
MarkerSeek score 20.20
Primer available yes
Label rpl23

Feature Metadata

label_name
rpl23
feature_type
gene
region
IRa
length_bp
282
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
20.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23_p1 GAATGGTTAAAGCGCCCAAC CACTTGACTGTCTACGGCTC 50.0 55.0 58.0 58.0 593 bp 1.0 80.1
rpl23_p2 GAATGGTTAAAGCGCCCAAC CCACAACGATGCTGTCCATA 50.0 50.0 58.0 58.0 640 bp 1.0 80.0
rpl23_p3 GAATGGTTAAAGCGCCCAAC CAATTCGCCTCTGACCATCA 50.0 50.0 58.0 58.0 1222 bp 1.0 79.8
rpl23_p4 CGATGAGCCCTGTTAAGCAT CACTTGACTGTCTACGGCTC 50.0 55.0 58.0 58.0 621 bp 1.0 79.8
rpl23_p5 GAATGGTTAAAGCGCCCAAC TGTGAATAACCCGTCCTCCT 50.0 50.0 58.0 58.1 1195 bp 1.0 79.6