Hotspot

rpl2

IRb · 83594-85073 · 1480 bp

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Pi 0
MarkerSeek score 25.20
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRb
length_bp
1480
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 CCCCCGCCAATGAATGATAA GAATGGTTAAAGCGCCCAAC 50.0 50.0 58.0 58.0 2591 bp 1.0 79.9
rpl2_p2 CATGTCTCGCGAAAGTCAGA GAATGGTTAAAGCGCCCAAC 50.0 50.0 58.0 58.0 2518 bp 1.0 79.9
rpl2_p3 TTTCCTCATAAGAACGCCCG GAATGGTTAAAGCGCCCAAC 50.0 50.0 58.0 58.0 2718 bp 1.0 79.8
rpl2_p4 CGCGCTTTGAAAACAGACAT GAATGGTTAAAGCGCCCAAC 45.0 50.0 58.0 58.0 2677 bp 1.0 79.7
rpl2_p5 CCCCCGCCAATGAATGATAA CGATGAGCCCTGTTAAGCAT 50.0 50.0 58.0 58.0 2619 bp 1.0 79.7