Hotspot

petD

LSC · 75649-76907 · 1259 bp

Back to Ruscus

Pi 0.00159
MarkerSeek score 59.70
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1259
variable_sites
2
pi
0.00159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 ACTTCTCTTCAACTCCGAAGT GGCACACGATCTGTATTTTCG 42.9 47.6 56.6 57.7 1429 bp 1.0 50.6
petD_p2 ACTTCTCTTCAACTCCGAAGT GCACACGATCTGTATTTTCGC 42.9 47.6 56.6 58.3 1428 bp 1.0 50.6
petD_p3 TGGATACTTCTCTTCAACTCCG GGCACACGATCTGTATTTTCG 45.5 47.6 57.3 57.7 1434 bp 1.0 47.9
petD_p4 TGGATACTTCTCTTCAACTCCG GCACACGATCTGTATTTTCGC 45.5 47.6 57.3 58.3 1433 bp 1.0 47.9
petD_p5 GGATACTTCTCTTCAACTCCGA GGCACACGATCTGTATTTTCG 45.5 47.6 57.0 57.7 1433 bp 1.0 45.8