Hotspot

ndhD

SSC · 114772-116277 · 1506 bp

Back to Ruscus

Pi 0.00133
MarkerSeek score 59.50
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1506
variable_sites
2
pi
0.00133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TGTGTTGTACAGCTCAAAAATCT ATTTTCTCGAGCACGGGTTT 34.8 45.0 56.9 58.1 1621 bp 1.0 46.5
ndhD_p2 TGTGTTGTACAGCTCAAAAATCT TTTCTCGAGCACGGGTTTTT 34.8 45.0 56.9 58.3 1619 bp 1.0 44.8
ndhD_p3 TGTGTTGTACAGCTCAAAAATCT TTTTCTCGAGCACGGGTTTT 34.8 45.0 56.9 58.3 1620 bp 1.0 44.8
ndhD_p4 TGTGTTGTACAGCTCAAAAA ATTTTCTCGAGCACGGGTTT 35.0 45.0 53.6 58.1 1621 bp 1.0 43.9
ndhD_p5 TTGTGTTGTACAGCTCAAAA ATTTTCTCGAGCACGGGTTT 35.0 45.0 53.6 58.1 1622 bp 1.0 43.9