Hotspot

atpA

LSC · 9178-10701 · 1524 bp

Back to Ruscus

Pi 0
MarkerSeek score 27.90
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 GGCGATACCAAGAAACCCTT TTTACCTTTGTTTCCGAAAAA 50.0 28.6 57.9 52.4 1649 bp 1.0 40.0
atpA_p2 GGCGATACCAAGAAACCCTT TTTTACCTTTGTTTCCGAAAA 50.0 28.6 57.9 52.4 1650 bp 1.0 40.0
atpA_p3 GGCGATACCAAGAAACCCTT TTTTTACCTTTGTTTCCGAAA 50.0 28.6 57.9 52.4 1651 bp 1.0 40.0
atpA_p4 GGCGATACCAAGAAACCCTT TTTTACCTTTGTTTCCGAAAAA 50.0 27.3 57.9 53.3 1650 bp 1.0 40.0
atpA_p5 GGCGATACCAAGAAACCCTT TTTTTACCTTTGTTTCCGAAAA 50.0 27.3 57.9 53.3 1651 bp 1.0 40.0