Hotspot

petA-psbJ

LSC · 62219-63281 · 1063 bp

Back to Ruscus

Pi 0
MarkerSeek score 61.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1063
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCCGTTATTTTGGCAC CCGGATTGGGTTCATCTCTG 50.0 55.0 58.1 58.0 1136-1171 bp 1.0 81.5
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC CCGGATTGGGTTCATCTCTG 50.0 55.0 57.8 58.0 1153-1188 bp 1.0 80.8
petA-psbJ_p3 GGCATCCGTTATTTTGGCAC TCCGGATTGGGTTCATCTCT 50.0 50.0 58.1 58.1 1137-1172 bp 1.0 80.8
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC TCCGGATTGGGTTCATCTCT 50.0 50.0 57.8 58.1 1154-1189 bp 1.0 80.2
petA-psbJ_p5 TGTTCTTCTTGGCATCCGTT CCGGATTGGGTTCATCTCTG 45.0 55.0 57.7 58.0 1146-1181 bp 1.0 79.9