Hotspot

ycf3-trnS-GGA

LSC · 43118-43690 · 573 bp

Back to Ruscus

Pi 0.00175
MarkerSeek score 59.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
573
variable_sites
1
pi
0.00175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTTCCCCTGAAGTTGTCGG ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.0 58.2 760 bp 1.0 78.7
ycf3-trnS-GGA_p2 TTTTCCCCTGAAGTTGTCGG AACGCCGGTGAATTAACAGA 50.0 45.0 58.0 57.8 781 bp 1.0 78.5
ycf3-trnS-GGA_p3 TTTTCCCCTGAAGTTGTCGG ACGCCGGTGAATTAACAGAA 50.0 45.0 58.0 57.8 780 bp 1.0 78.5
ycf3-trnS-GGA_p4 TTTTCCCCTGAAGTTGTCGG TTCCAATGCTACGCCTTGAA 50.0 45.0 58.0 57.8 709 bp 1.0 78.4
ycf3-trnS-GGA_p5 CCCCTGAAGTTGTCGGAATT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 756 bp 1.0 77.3