Hotspot

rpoB-trnC-GCA

LSC · 25606-26846 · 1241 bp

Back to Ruscus

Pi 0.0146
MarkerSeek score 67.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1241
variable_sites
18
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGACAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 1389 bp 1.0 75.8
rpoB-trnC-GCA_p2 AATTCTTCCGACAAGCCCTG TTTGTTGATCAGGCGACACC 50.0 50.0 58.2 58.8 1426 bp 1.0 73.8
rpoB-trnC-GCA_p3 AATTCTTCCGACAAGCCCTG GACACCCGGATTTGAACTGG 50.0 55.0 58.2 58.8 1412 bp 1.0 73.3
rpoB-trnC-GCA_p4 AAATTCTTCCGACAAGCCCT TAAAGGATTTGCAGTCCCCC 45.0 50.0 57.4 57.5 1390 bp 1.0 72.5
rpoB-trnC-GCA_p5 AATTCTTCCGACAAGCCCTG CCGGATTTGAACTGGGGATA 50.0 50.0 58.2 57.0 1407 bp 1.0 71.8