primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU_p1 trnK-UUU 1 TGAAGCGAGCTAAAACTCAA ACGTAGTTGTACAGTGCAAT 20 20 40.0 40.0 55.3 55.0 0.0 7.4 5.633724 1375 4024 2776-2776 1.000000 40.0 trnK-UUU_p2 trnK-UUU 2 TTGAAGCGAGCTAAAACTCA ACGTAGTTGTACAGTGCAAT 20 20 40.0 40.0 55.3 55.0 0.0 7.4 5.633724 1375 4024 2777-2777 1.000000 40.0 trnK-UUU_p3 trnK-UUU 3 TGAAGCGAGCTAAAACTCAA CGTAGTTGTACAGTGCAATTCA 20 22 40.0 40.9 55.3 57.0 0.0 7.4 5.654663 1375 4024 2775-2775 1.000000 40.0 trnK-UUU_p4 trnK-UUU 4 TTGAAGCGAGCTAAAACTCA CGTAGTTGTACAGTGCAATTCA 20 22 40.0 40.9 55.3 57.0 0.0 7.4 5.654663 1375 4024 2776-2776 1.000000 40.0 trnK-UUU_p5 trnK-UUU 5 TGAAGCGAGCTAAAACTCAA ACGTAGTTGTACAGTGCAATTC 20 22 40.0 40.9 55.3 57.0 0.0 7.4 5.656492 1375 4024 2776-2776 1.000000 40.0 trnK-UUU-trnQ-UUG_p1 trnK-UUU-trnQ-UUG 1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 20.3 16.9 0.244521 4025 5711 1745-1745 1.000000 78.1 trnK-UUU-trnQ-UUG_p2 trnK-UUU-trnQ-UUG 2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 20.3 16.9 0.244521 4025 5711 1744-1744 1.000000 78.1 trnK-UUU-trnQ-UUG_p3 trnK-UUU-trnQ-UUG 3 TACCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.5 58.1 0.0 16.9 0.536858 4025 5711 1731-1731 1.000000 75.8 trnK-UUU-trnQ-UUG_p4 trnK-UUU-trnQ-UUG 4 AGTCGCACTTAAAAGCCGAG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.6 58.1 6.4 16.9 0.646638 4025 5711 1756-1756 1.000000 74.9 trnK-UUU-trnQ-UUG_p5 trnK-UUU-trnQ-UUG 5 TCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 57.3 58.1 3.0 16.9 0.804791 4025 5711 1754-1754 1.000000 73.6 atpA_p1 atpA 1 GGCGATACCAAGAAACCCTT TTTACCTTTGTTTCCGAAAAA 20 21 50.0 28.6 57.9 52.4 0.0 0.0 6.765043 9178 10701 1649-1649 1.000000 40.0 atpA_p2 atpA 2 GGCGATACCAAGAAACCCTT TTTTACCTTTGTTTCCGAAAA 20 21 50.0 28.6 57.9 52.4 0.0 0.0 6.765043 9178 10701 1650-1650 1.000000 40.0 atpA_p3 atpA 3 GGCGATACCAAGAAACCCTT TTTTTACCTTTGTTTCCGAAA 20 21 50.0 28.6 57.9 52.4 0.0 0.0 6.765043 9178 10701 1651-1651 1.000000 40.0 atpA_p4 atpA 4 GGCGATACCAAGAAACCCTT TTTTACCTTTGTTTCCGAAAAA 20 22 50.0 27.3 57.9 53.3 0.0 0.0 6.864227 9178 10701 1650-1650 1.000000 40.0 atpA_p5 atpA 5 GGCGATACCAAGAAACCCTT TTTTTACCTTTGTTTCCGAAAA 20 22 50.0 27.3 57.9 53.3 0.0 0.0 6.864227 9178 10701 1651-1651 1.000000 40.0 atpA-atpF_p1 atpA-atpF 1 CCGTCGCCTACTTGAAGTAC CGGGGCCATGGAAGAAATAA 20 20 55.0 50.0 58.1 57.9 0.0 18.4 0.160449 10702 10775 215-215 1.000000 79.1 atpA-atpF_p2 atpA-atpF 2 GTCGCCTACTTGAAGTACGG CGGGGCCATGGAAGAAATAA 20 20 55.0 50.0 58.1 57.9 13.2 18.4 0.160449 10702 10775 213-213 1.000000 79.1 atpA-atpF_p3 atpA-atpF 3 CCGTCGCCTACTTGAAGTAC AGCCTTACAGGGAGCTCTAG 20 20 55.0 55.0 58.1 57.9 0.0 14.6 0.175204 10702 10775 302-302 1.000000 78.9 atpA-atpF_p4 atpA-atpF 4 GTCGCCTACTTGAAGTACGG AGCCTTACAGGGAGCTCTAG 20 20 55.0 55.0 58.1 57.9 13.2 14.6 0.175204 10702 10775 300-300 1.000000 78.9 atpA-atpF_p5 atpA-atpF 5 GTCGCCTACTTGAAGTACGG GCGAGTTACATCTCCGTACC 20 20 55.0 55.0 58.1 57.9 13.2 0.0 0.211338 10702 10775 256-256 1.000000 78.6 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 AATTCTTCCGACAAGCCCTG TAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 58.2 57.5 0.0 14.6 0.698720 25606 26846 1389-1389 1.000000 75.8 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 AATTCTTCCGACAAGCCCTG TTTGTTGATCAGGCGACACC 20 20 50.0 50.0 58.2 58.8 0.0 0.0 0.938794 25606 26846 1426-1426 1.000000 73.8 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 AATTCTTCCGACAAGCCCTG GACACCCGGATTTGAACTGG 20 20 50.0 55.0 58.2 58.8 0.0 0.7 1.008345 25606 26846 1412-1412 1.000000 73.3 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 AAATTCTTCCGACAAGCCCT TAAAGGATTTGCAGTCCCCC 20 20 45.0 50.0 57.4 57.5 0.0 14.6 1.111753 25606 26846 1390-1390 1.000000 72.5 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 AATTCTTCCGACAAGCCCTG CCGGATTTGAACTGGGGATA 20 20 50.0 50.0 58.2 57.0 0.0 0.0 1.194840 25606 26846 1407-1407 1.000000 71.8 trnC-GCA-petN_p1 trnC-GCA-petN 1 ATTTTGGACCGAATTTGGCG TTAAAGCAGCCCAAGCAAGA 20 20 45.0 45.0 57.6 58.0 12.0 0.0 0.368213 26918 27837 1041-1041 1.000000 77.1 trnC-GCA-petN_p2 trnC-GCA-petN 2 ATTTTGGACCGAATTTGGCG AGCAATTAGTAGTGCCCAGC 20 20 45.0 50.0 57.6 58.0 12.0 4.7 0.406292 26918 27837 1118-1118 1.000000 76.8 trnC-GCA-petN_p3 trnC-GCA-petN 3 ATTTTGGACCGAATTTGGCG AGTCCACTCCTTCCCCATAC 20 20 45.0 55.0 57.6 58.1 12.0 0.0 0.481229 26918 27837 1093-1093 1.000000 76.2 trnC-GCA-petN_p4 trnC-GCA-petN 4 ATTTTGGACCGAATTTGGCG GTCCACTCCTTCCCCATACT 20 20 45.0 55.0 57.6 58.1 12.0 0.0 0.481229 26918 27837 1092-1092 1.000000 76.2 trnC-GCA-petN_p5 trnC-GCA-petN 5 GGGGGACTGCAAATCCTTTA TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 57.5 58.0 14.7 0.0 0.531149 26918 27837 1001-1001 1.000000 75.8 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 20 20 55.0 50.0 58.1 58.1 7.6 0.0 0.177181 30831 31593 923-924 1.000000 78.7 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.199336 30831 31593 926-927 1.000000 78.5 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.3 58.1 0.0 0.0 0.395920 30831 31593 900-901 1.000000 76.9 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GGCCCTTTTAACTCAGTGGT GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 57.7 58.1 0.0 0.0 0.401091 30831 31593 938-939 1.000000 76.9 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 TGGTAGAGTAACGCCATGGT GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.4 58.1 14.7 0.0 0.549189 30831 31593 922-923 1.000000 75.7 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 TTTTCCCCTGAAGTTGTCGG ACGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.175689 43118 43690 760-760 1.000000 78.7 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 TTTTCCCCTGAAGTTGTCGG AACGCCGGTGAATTAACAGA 20 20 50.0 45.0 58.0 57.8 0.0 24.6 0.197191 43118 43690 781-781 1.000000 78.5 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 TTTTCCCCTGAAGTTGTCGG ACGCCGGTGAATTAACAGAA 20 20 50.0 45.0 58.0 57.8 0.0 24.6 0.197191 43118 43690 780-780 1.000000 78.5 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 TTTTCCCCTGAAGTTGTCGG TTCCAATGCTACGCCTTGAA 20 20 50.0 45.0 58.0 57.8 0.0 5.6 0.217004 43118 43690 709-709 1.000000 78.4 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 CCCCTGAAGTTGTCGGAATT ACGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 57.8 58.2 0.0 0.0 0.351721 43118 43690 756-756 1.000000 77.3 trnV-UAC_p1 trnV-UAC 1 CAAAGGAACCTGTCAGTCCA TGTGGGGTCCTATCTTGACA 20 20 50.0 50.0 57.4 57.7 11.1 0.0 0.974161 50745 51414 820-820 1.000000 72.3 trnV-UAC_p2 trnV-UAC 2 TGGACAAAGGAACCTGTCAG TGTGGGGTCCTATCTTGACA 20 20 50.0 50.0 57.4 57.7 12.8 0.0 0.974161 50745 51414 824-824 1.000000 72.3 trnV-UAC_p3 trnV-UAC 3 ACAAAGGAACCTGTCAGTCC TGTGGGGTCCTATCTTGACA 20 20 50.0 50.0 57.4 57.7 0.0 0.0 0.975997 50745 51414 821-821 1.000000 72.3 trnV-UAC_p4 trnV-UAC 4 GGACAAAGGAACCTGTCAGT TGTGGGGTCCTATCTTGACA 20 20 50.0 50.0 57.4 57.7 12.8 0.0 0.975997 50745 51414 823-823 1.000000 72.3 trnV-UAC_p5 trnV-UAC 5 CAAAGGAACCTGTCAGTCCA AGAGAGCTATGATGTGGGGT 20 20 50.0 50.0 57.4 57.5 11.1 0.0 1.124446 50745 51414 832-832 1.000000 71.1 petA-psbJ_p1 petA-psbJ 1 GGCATCCGTTATTTTGGCAC CCGGATTGGGTTCATCTCTG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.119715 62219 63281 1136-1171 1.000000 81.5 petA-psbJ_p2 petA-psbJ 2 GGCCTTTTGTTCTTCTTGGC CCGGATTGGGTTCATCTCTG 20 20 50.0 55.0 57.8 58.0 0.0 0.0 0.195812 62219 63281 1153-1188 1.000000 80.8 petA-psbJ_p3 petA-psbJ 3 GGCATCCGTTATTTTGGCAC TCCGGATTGGGTTCATCTCT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.199182 62219 63281 1137-1172 1.000000 80.8 petA-psbJ_p4 petA-psbJ 4 GGCCTTTTGTTCTTCTTGGC TCCGGATTGGGTTCATCTCT 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.275279 62219 63281 1154-1189 1.000000 80.2 petA-psbJ_p5 petA-psbJ 5 TGTTCTTCTTGGCATCCGTT CCGGATTGGGTTCATCTCTG 20 20 45.0 55.0 57.7 58.0 0.0 0.0 0.311310 62219 63281 1146-1181 1.000000 79.9 petD_p1 petD 1 ACTTCTCTTCAACTCCGAAGT GGCACACGATCTGTATTTTCG 21 21 42.9 47.6 56.6 57.7 0.0 0.0 3.689645 75649 76907 1429-1429 1.000000 50.6 petD_p2 petD 2 ACTTCTCTTCAACTCCGAAGT GCACACGATCTGTATTTTCGC 21 21 42.9 47.6 56.6 58.3 0.0 0.0 3.691220 75649 76907 1428-1428 1.000000 50.6 petD_p3 petD 3 TGGATACTTCTCTTCAACTCCG GGCACACGATCTGTATTTTCG 22 21 45.5 47.6 57.3 57.7 0.0 0.0 4.025032 75649 76907 1434-1434 1.000000 47.9 petD_p4 petD 4 TGGATACTTCTCTTCAACTCCG GCACACGATCTGTATTTTCGC 22 21 45.5 47.6 57.3 58.3 0.0 0.0 4.026607 75649 76907 1433-1433 1.000000 47.9 petD_p5 petD 5 GGATACTTCTCTTCAACTCCGA GGCACACGATCTGTATTTTCG 22 21 45.5 47.6 57.0 57.7 0.0 0.0 4.289920 75649 76907 1433-1433 1.000000 45.8 rpl2_p1 rpl2 1 CCCCCGCCAATGAATGATAA GAATGGTTAAAGCGCCCAAC 20 20 50.0 50.0 58.0 58.0 0.0 2.3 0.015245 83594 85073 2591-2591 1.000000 79.9 rpl2_p2 rpl2 2 CATGTCTCGCGAAAGTCAGA GAATGGTTAAAGCGCCCAAC 20 20 50.0 50.0 58.0 58.0 11.3 2.3 0.026589 83594 85073 2518-2518 1.000000 79.9 rpl2_p3 rpl2 3 TTTCCTCATAAGAACGCCCG GAATGGTTAAAGCGCCCAAC 20 20 50.0 50.0 58.0 58.0 0.0 2.3 0.027743 83594 85073 2718-2718 1.000000 79.8 rpl2_p4 rpl2 4 CGCGCTTTGAAAACAGACAT GAATGGTTAAAGCGCCCAAC 20 20 45.0 50.0 58.0 58.0 5.6 2.3 0.045757 83594 85073 2677-2677 1.000000 79.7 rpl2_p5 rpl2 5 CCCCCGCCAATGAATGATAA CGATGAGCCCTGTTAAGCAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.047002 83594 85073 2619-2619 1.000000 79.7 rpl2-rpl23_p1 rpl2-rpl23 1 CACTTGACTGTCTACGGCTC CGCTTCAACCGGGTTATTCT 20 20 55.0 50.0 58.0 58.3 9.5 13.0 0.281865 85074 85097 134-134 1.000000 78.9 rpl2-rpl23_p2 rpl2-rpl23 2 CCACAACGATGCTGTCCATA CGCTTCAACCGGGTTATTCT 20 20 50.0 50.0 58.0 58.3 0.0 13.0 0.288602 85074 85097 181-181 1.000000 78.6 rpl2-rpl23_p3 rpl2-rpl23 3 GACTGTCTACGGCTCCATTG CGCTTCAACCGGGTTATTCT 20 20 55.0 50.0 58.1 58.3 0.0 13.0 0.337942 85074 85097 129-129 1.000000 78.5 rpl2-rpl23_p4 rpl2-rpl23 4 CACTTGACTGTCTACGGCTC ATCATTACGCTTCAACCGGG 20 20 55.0 50.0 58.0 58.3 9.5 6.3 0.352333 85074 85097 141-141 1.000000 78.3 rpl2-rpl23_p5 rpl2-rpl23 5 CCACAACGATGCTGTCCATA ATCATTACGCTTCAACCGGG 20 20 50.0 50.0 58.0 58.3 0.0 6.3 0.359070 85074 85097 188-188 1.000000 78.0 rps12_p1 rps12 1 TGACATGAGTGCGAAAGAGG ATTGAACGAGGAGCCGTATG 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.309678 96913 97712 949-949 1.000000 77.6 rps12_p2 rps12 2 GACATGAGTGCGAAAGAGGT ATTGAACGAGGAGCCGTATG 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.311123 96913 97712 948-948 1.000000 77.6 rps12_p3 rps12 3 CAGTACCTCGACGTGACATG ATTGAACGAGGAGCCGTATG 20 20 55.0 50.0 58.1 57.8 0.0 0.0 0.328723 96913 97712 962-962 1.000000 77.5 rps12_p4 rps12 4 TGACATGAGTGCGAAAGAGG AAGAATTGAACGAGGAGCCG 20 20 50.0 50.0 57.9 58.3 0.0 0.0 0.357756 96913 97712 953-953 1.000000 77.2 rps12_p5 rps12 5 GACATGAGTGCGAAAGAGGT AAGAATTGAACGAGGAGCCG 20 20 50.0 50.0 57.9 58.3 0.0 0.0 0.359201 96913 97712 952-952 1.000000 77.2 ndhD_p1 ndhD 1 TGTGTTGTACAGCTCAAAAATCT ATTTTCTCGAGCACGGGTTT 23 20 34.8 45.0 56.9 58.1 11.4 7.8 4.195518 114772 116277 1621-1621 1.000000 46.5 ndhD_p2 ndhD 2 TGTGTTGTACAGCTCAAAAATCT TTTCTCGAGCACGGGTTTTT 23 20 34.8 45.0 56.9 58.3 11.4 7.8 4.416937 114772 116277 1619-1619 1.000000 44.8 ndhD_p3 ndhD 3 TGTGTTGTACAGCTCAAAAATCT TTTTCTCGAGCACGGGTTTT 23 20 34.8 45.0 56.9 58.3 11.4 7.8 4.416937 114772 116277 1620-1620 1.000000 44.8 ndhD_p4 ndhD 4 TGTGTTGTACAGCTCAAAAA ATTTTCTCGAGCACGGGTTT 20 20 35.0 45.0 53.6 58.1 11.4 7.8 4.529340 114772 116277 1621-1621 1.000000 43.9 ndhD_p5 ndhD 5 TTGTGTTGTACAGCTCAAAA ATTTTCTCGAGCACGGGTTT 20 20 35.0 45.0 53.6 58.1 20.3 7.8 4.529340 114772 116277 1622-1622 1.000000 43.9 rps12_p1 rps12 1 ATTGAACGAGGAGCCGTATG TGACATGAGTGCGAAAGAGG 20 20 50.0 50.0 57.8 57.9 0.0 0.0 0.309678 139179 139978 949-949 1.000000 77.6 rps12_p2 rps12 2 ATTGAACGAGGAGCCGTATG GACATGAGTGCGAAAGAGGT 20 20 50.0 50.0 57.8 57.9 0.0 0.0 0.311123 139179 139978 948-948 1.000000 77.6 rps12_p3 rps12 3 ATTGAACGAGGAGCCGTATG CAGTACCTCGACGTGACATG 20 20 50.0 55.0 57.8 58.1 0.0 0.0 0.328723 139179 139978 962-962 1.000000 77.5 rps12_p4 rps12 4 AAGAATTGAACGAGGAGCCG TGACATGAGTGCGAAAGAGG 20 20 50.0 50.0 58.3 57.9 0.0 0.0 0.357756 139179 139978 953-953 1.000000 77.2 rps12_p5 rps12 5 AAGAATTGAACGAGGAGCCG GACATGAGTGCGAAAGAGGT 20 20 50.0 50.0 58.3 57.9 0.0 0.0 0.359201 139179 139978 952-952 1.000000 77.2 rpl23_p1 rpl23 1 GAATGGTTAAAGCGCCCAAC CACTTGACTGTCTACGGCTC 20 20 50.0 55.0 58.0 58.0 2.3 9.5 0.025228 151512 151793 593-593 1.000000 80.1 rpl23_p2 rpl23 2 GAATGGTTAAAGCGCCCAAC CCACAACGATGCTGTCCATA 20 20 50.0 50.0 58.0 58.0 2.3 0.0 0.031965 151512 151793 640-640 1.000000 80.0 rpl23_p3 rpl23 3 GAATGGTTAAAGCGCCCAAC CAATTCGCCTCTGACCATCA 20 20 50.0 50.0 58.0 58.0 2.3 0.0 0.037621 151512 151793 1222-1222 1.000000 79.8 rpl23_p4 rpl23 4 CGATGAGCCCTGTTAAGCAT CACTTGACTGTCTACGGCTC 20 20 50.0 55.0 58.0 58.0 0.0 9.5 0.056986 151512 151793 621-621 1.000000 79.8 rpl23_p5 rpl23 5 GAATGGTTAAAGCGCCCAAC TGTGAATAACCCGTCCTCCT 20 20 50.0 50.0 58.0 58.1 2.3 0.0 0.062216 151512 151793 1195-1195 1.000000 79.6 rpl23-rpl2_p1 rpl23-rpl2 1 CGCTTCAACCGGGTTATTCT CACTTGACTGTCTACGGCTC 20 20 50.0 55.0 58.3 58.0 13.0 9.5 0.281865 151794 151817 134-134 1.000000 78.9 rpl23-rpl2_p2 rpl23-rpl2 2 CGCTTCAACCGGGTTATTCT CCACAACGATGCTGTCCATA 20 20 50.0 50.0 58.3 58.0 13.0 0.0 0.288602 151794 151817 181-181 1.000000 78.6 rpl23-rpl2_p3 rpl23-rpl2 3 CGCTTCAACCGGGTTATTCT GACTGTCTACGGCTCCATTG 20 20 50.0 55.0 58.3 58.1 13.0 0.0 0.337942 151794 151817 129-129 1.000000 78.5 rpl23-rpl2_p4 rpl23-rpl2 4 ATCATTACGCTTCAACCGGG CACTTGACTGTCTACGGCTC 20 20 50.0 55.0 58.3 58.0 6.3 9.5 0.352333 151794 151817 141-141 1.000000 78.3 rpl23-rpl2_p5 rpl23-rpl2 5 ATCATTACGCTTCAACCGGG CCACAACGATGCTGTCCATA 20 20 50.0 50.0 58.3 58.0 6.3 0.0 0.359070 151794 151817 188-188 1.000000 78.0