Hotspot

ycf1

SSC · 129089-134413 · 5325 bp

Pi 0.0122

MarkerSeek score 58.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5325
variable_sites: 97
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATTTTGGTGGACGAGGT	ACAATTTTGTTGCCAGTAAACCA	45.0	34.8	57.4	57.9	216 bp	1.0	53.0
ycf1_p2	GTCAATTTTGGTGGACGAGG	ACAATTTTGTTGCCAGTAAACCA	50.0	34.8	57.0	57.9	217 bp	1.0	50.3
ycf1_p3	CCTTGTCAATTTTGGTGGACG	ACAATTTTGTTGCCAGTAAACCA	47.6	34.8	58.0	57.9	221 bp	1.0	50.1
ycf1_p4	TGGTGGACGAGGTACTTTTT	ACAATTTTGTTGCCAGTAAACCA	45.0	34.8	56.7	57.9	209 bp	1.0	47.9
ycf1_p5	TCAATTTTGGTGGACGAGGT	CAATTTTGTTGCCAGTAAACCA	45.0	36.4	57.4	56.3	215 bp	1.0	47.8