Hotspot

rps16

LSC · 5169-6521 · 1353 bp

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Pi 0.00908
MarkerSeek score 54.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1353
variable_sites
18
pi
0.00908
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGTTGTCCTAATCCACCTTT TGTACCGATCGAGCTAATGAC 40.0 47.6 53.8 57.4 1444-1473 bp 1.0 42.7
rps16_p2 AGTTGTCCTAATCCACCTTTT TGTACCGATCGAGCTAATGAC 38.1 47.6 54.7 57.4 1444-1473 bp 1.0 42.7
rps16_p3 AGTTGTCCTAATCCACCTTT GTACCGATCGAGCTAATGACT 40.0 47.6 53.8 57.1 1443-1472 bp 1.0 42.7
rps16_p4 AGTTGTCCTAATCCACCTTTTT TGTACCGATCGAGCTAATGAC 36.4 47.6 55.5 57.4 1444-1473 bp 1.0 42.7
rps16_p5 AGTTGTCCTAATCCACCTTTT GTACCGATCGAGCTAATGACT 38.1 47.6 54.7 57.1 1443-1472 bp 1.0 42.7