Hotspot

ccsA-ndhD

SSC · 119804-120036 · 233 bp

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Pi 0.0295
MarkerSeek score 64.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
233
variable_sites
10
pi
0.0295
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGGCAATTGTAGCTTCTATGG TGGTTCTCTCGCTATCAGTTG 47.6 47.6 57.1 57.5 404-411 bp 1.0 56.2
ccsA-ndhD_p2 CGGCAATTGTAGCTTCTATGG GGTTCTCTCGCTATCAGTTGA 47.6 47.6 57.1 57.2 403-410 bp 1.0 54.0
ccsA-ndhD_p3 GGCAATTGTAGCTTCTATGGGA TGGTTCTCTCGCTATCAGTTG 45.5 47.6 58.2 57.5 403-410 bp 1.0 53.8
ccsA-ndhD_p4 GGCAATTGTAGCTTCTATGGG TGGTTCTCTCGCTATCAGTTG 47.6 47.6 56.7 57.5 403-410 bp 1.0 53.1
ccsA-ndhD_p5 TGAATTCGGCAATTGTAGCT TGGTTCTCTCGCTATCAGTTG 40.0 47.6 55.7 57.5 410-417 bp 1.0 53.0