Hotspot

rpl32-trnL-UAG

SSC · 117953-118649 · 697 bp

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Pi 0.0234
MarkerSeek score 63.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
697
variable_sites
22
pi
0.0234
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCAAATCAATTTCCACGGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 747-796 bp 1.0 85.3
rpl32-trnL-UAG_p2 GCCAAATCAATTTCCACGGG ACGTTTCGGTTGAAAGACCA 50.0 45.0 58.0 58.0 1265-1321 bp 1.0 85.2
rpl32-trnL-UAG_p3 GCCAAATCAATTTCCACGGG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 746-795 bp 1.0 84.8
rpl32-trnL-UAG_p4 GCCAAATCAATTTCCACGGG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 745-794 bp 1.0 84.8
rpl32-trnL-UAG_p5 GCCAAATCAATTTCCACGGG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 789 bp 0.333333 51.8