Hotspot

ndhF-rpl32

SSC · 116653-117790 · 1138 bp

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Pi 0.0217
MarkerSeek score 62.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1138
variable_sites
36
pi
0.0217
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CATTCCCACCAGTTCCCAAA CCCGTGGAAATTGATTTGGC 50.0 50.0 58.0 58.0 1723-1728 bp 1.0 82.6
ndhF-rpl32_p2 AATACGAGCACATTCCCACC CCCGTGGAAATTGATTTGGC 50.0 50.0 58.0 58.0 1733-1738 bp 1.0 82.3
ndhF-rpl32_p3 CGGTCGTGTGAACCAAAAAC CCCGTGGAAATTGATTTGGC 50.0 50.0 58.2 58.0 1762-1767 bp 1.0 81.1
ndhF-rpl32_p4 GGTCGTGTGAACCAAAAACC CCCGTGGAAATTGATTTGGC 50.0 50.0 57.8 58.0 1761-1766 bp 1.0 81.0
ndhF-rpl32_p5 TTGTGCGGATTTTGCGATTG CCCGTGGAAATTGATTTGGC 45.0 50.0 58.3 58.0 1984-1989 bp 1.0 80.0