Hotspot

petN-psbM

LSC · 31242-32296 · 1055 bp

Back to Ruppia

Pi 0.0114
MarkerSeek score 65.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1055
variable_sites
18
pi
0.0114
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TTTCCCTTTCGCTCGTAGTG CGTAAAAACAGTCAGTCAAAAGGA 50.0 37.5 57.9 57.9 1124-1136 bp 1.0 47.8
petN-psbM_p2 TTTCCCTTTCGCTCGTAGTG ACGTAAAAACAGTCAGTCAAAAGG 50.0 37.5 57.9 58.1 1125-1137 bp 1.0 47.8
petN-psbM_p3 ACATTTTCCCTTTCGCTCGT CGTAAAAACAGTCAGTCAAAAGGA 45.0 37.5 58.1 57.9 1128-1140 bp 1.0 47.6
petN-psbM_p4 ACATTTTCCCTTTCGCTCGT ACGTAAAAACAGTCAGTCAAAAGG 45.0 37.5 58.1 58.1 1129-1141 bp 1.0 47.5
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT CGTAAAAACAGTCAGTCAAAAGGA 50.0 37.5 58.2 57.9 1166-1178 bp 1.0 47.2