Hotspot

trnQ-UUG-psbK

LSC · 8184-8873 · 690 bp

Back to Ruppia

Pi 0.0117
MarkerSeek score 66.30
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
690
variable_sites
12
pi
0.0117
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 TCAAAACCGGTTGCCTTACC GGGCAATTTGGCGAAGAAAA 50.0 45.0 58.7 57.8 797-809 bp 1.0 78.1
trnQ-UUG-psbK_p2 AAAACCGGTTGCCTTACCAC GGGCAATTTGGCGAAGAAAA 50.0 45.0 59.0 57.8 795-807 bp 1.0 75.9
trnQ-UUG-psbK_p3 CGAACCTCCGAATAACAGGA GGGCAATTTGGCGAAGAAAA 50.0 45.0 57.0 57.8 817-829 bp 1.0 75.8
trnQ-UUG-psbK_p4 TCGAACCTCCGAATAACAGG GGGCAATTTGGCGAAGAAAA 50.0 45.0 57.0 57.8 818-830 bp 1.0 75.8
trnQ-UUG-psbK_p5 CAAAACCGGTTGCCTTACCA GGGCAATTTGGCGAAGAAAA 50.0 45.0 59.0 57.8 796-808 bp 1.0 75.8