primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	43	363	442-445	1.000000	79.9
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.307703	43	363	444-447	1.000000	79.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306133	43	363	456-459	1.000000	79.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	43	363	455-458	1.000000	79.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	43	363	457-460	1.000000	79.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	CAACCCACAGAGACGGTTTA	20	20	50.0	50.0	58.6	57.5	6.4	0.0	1.107298	4167	5168	1125-1134	1.000000	73.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TAGTCGCACTTAAAAGCCGA	CAACCCACAGAGACGGTTTA	20	20	45.0	50.0	57.3	57.5	3.0	0.0	1.265451	4167	5168	1126-1135	1.000000	72.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCGCACTTAAAAGCCGAGTA	CAACCCACAGAGACGGTTTA	20	20	45.0	50.0	57.3	57.5	3.0	0.0	1.265451	4167	5168	1123-1132	1.000000	72.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGCACTTAAAAGCCGAGTAC	CAACCCACAGAGACGGTTTA	20	20	50.0	50.0	57.2	57.5	0.0	0.0	1.318959	4167	5168	1122-1131	1.000000	71.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	ACCCACAGAGACGGTTTATG	20	20	50.0	50.0	58.6	57.2	6.4	0.0	1.333073	4167	5168	1123-1132	1.000000	71.7
rps16_p1	rps16	1	AGTTGTCCTAATCCACCTTT	TGTACCGATCGAGCTAATGAC	20	21	40.0	47.6	53.8	57.4	0.0	18.7	5.801016	5169	6521	1444-1473	1.000000	42.7
rps16_p2	rps16	2	AGTTGTCCTAATCCACCTTTT	TGTACCGATCGAGCTAATGAC	21	21	38.1	47.6	54.7	57.4	0.0	18.7	5.906688	5169	6521	1444-1473	1.000000	42.7
rps16_p3	rps16	3	AGTTGTCCTAATCCACCTTT	GTACCGATCGAGCTAATGACT	20	21	40.0	47.6	53.8	57.1	0.0	18.7	6.080475	5169	6521	1443-1472	1.000000	42.7
rps16_p4	rps16	4	AGTTGTCCTAATCCACCTTTTT	TGTACCGATCGAGCTAATGAC	22	21	36.4	47.6	55.5	57.4	0.0	18.7	6.094728	5169	6521	1444-1473	1.000000	42.7
rps16_p5	rps16	5	AGTTGTCCTAATCCACCTTTT	GTACCGATCGAGCTAATGACT	21	21	38.1	47.6	54.7	57.1	0.0	18.7	6.186147	5169	6521	1443-1472	1.000000	42.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GCCAAGAGCATTTTCGTTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.093489	6522	8111	1729-1786	1.000000	81.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6522	8111	1663-1720	1.000000	80.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TGATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6522	8111	1674-1731	1.000000	79.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GCCAAGAGCATTTTCGTTCC	TAAATCGAAATGGGGCGTGG	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.632863	6522	8111	1787-1844	1.000000	76.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GCCAAGAGCATTTTCGTTCC	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	58.0	57.3	0.0	16.9	0.684861	6522	8111	1733-1790	1.000000	76.6
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	TCAAAACCGGTTGCCTTACC	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	58.7	57.8	23.1	15.8	0.841812	8184	8873	797-809	1.000000	78.1
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	AAAACCGGTTGCCTTACCAC	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	59.0	57.8	23.1	15.8	1.127963	8184	8873	795-807	1.000000	75.9
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	CGAACCTCCGAATAACAGGA	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	57.0	57.8	0.0	15.8	1.115785	8184	8873	817-829	1.000000	75.8
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	TCGAACCTCCGAATAACAGG	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	57.0	57.8	0.0	15.8	1.115785	8184	8873	818-830	1.000000	75.8
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	CAAAACCGGTTGCCTTACCA	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	59.0	57.8	23.1	15.8	1.128745	8184	8873	796-808	1.000000	75.8
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.060677	9060	9536	790-828	1.000000	85.1
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.062065	9060	9536	791-829	1.000000	85.1
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	CCGGGGGAATCAAAAAGGAA	20	20	50.0	50.0	57.9	58.1	1.4	0.0	0.124459	9060	9536	972-1016	1.000000	84.5
psbK-psbI_p4	psbK-psbI	4	GTTTGGCAAGCTGCTGTAAG	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.9	58.1	1.4	0.0	0.165542	9060	9536	809-847	1.000000	84.2
psbK-psbI_p5	psbK-psbI	5	TTTTCTTCGCCAAATTGCCC	TGGACTAAAGCGTCGGATTG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.166902	9060	9536	885-923	1.000000	84.1
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATGATCCCGGACGTAATCCT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.015705	9648	9784	243-245	1.000000	83.5
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATGATCCCGGACGTAATCCT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.017093	9648	9784	244-246	1.000000	83.5
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATGATCCCGGACGTAATCCT	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.0	58.2	0.7	17.2	0.228051	9648	9784	193-195	1.000000	82.7
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	ATGATCCCGGACGTAATCCT	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.0	58.1	0.7	0.0	0.120570	9648	9784	262-264	1.000000	82.4
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATGATCCCGGACGTAATCCT	TTCGAATCCCTCTCTCTCCG	20	20	50.0	55.0	58.0	58.3	0.7	0.0	0.329584	9648	9784	190-192	1.000000	82.0
petN-psbM_p1	petN-psbM	1	TTTCCCTTTCGCTCGTAGTG	CGTAAAAACAGTCAGTCAAAAGGA	20	24	50.0	37.5	57.9	57.9	0.0	0.0	4.188768	31242	32296	1124-1136	1.000000	47.8
petN-psbM_p2	petN-psbM	2	TTTCCCTTTCGCTCGTAGTG	ACGTAAAAACAGTCAGTCAAAAGG	20	24	50.0	37.5	57.9	58.1	0.0	0.0	4.198616	31242	32296	1125-1137	1.000000	47.8
petN-psbM_p3	petN-psbM	3	ACATTTTCCCTTTCGCTCGT	CGTAAAAACAGTCAGTCAAAAGGA	20	24	45.0	37.5	58.1	57.9	0.0	0.0	4.222012	31242	32296	1128-1140	1.000000	47.6
petN-psbM_p4	petN-psbM	4	ACATTTTCCCTTTCGCTCGT	ACGTAAAAACAGTCAGTCAAAAGG	20	24	45.0	37.5	58.1	58.1	0.0	0.0	4.231860	31242	32296	1129-1141	1.000000	47.5
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	CGTAAAAACAGTCAGTCAAAAGGA	20	24	50.0	37.5	58.2	57.9	0.0	0.0	4.265570	31242	32296	1166-1178	1.000000	47.2
psaA-ycf3_p1	psaA-ycf3	1	GTTTTTGAGAAATGCCCCGG	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.177990	45148	45845	910-915	1.000000	80.1
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.193903	45148	45845	885-890	1.000000	80.0
psaA-ycf3_p3	psaA-ycf3	3	GTTTTTGAGAAATGCCCCGG	ATAGCGCTTACTCCGGGTAA	20	20	50.0	50.0	57.9	58.3	0.0	18.7	0.382785	45148	45845	866-871	1.000000	78.5
psaA-ycf3_p4	psaA-ycf3	4	GTTTTTGAGAAATGCCCCGG	TAGCGCTTACTCCGGGTAAT	20	20	50.0	50.0	57.9	58.3	0.0	18.7	0.382785	45148	45845	865-870	1.000000	78.5
psaA-ycf3_p5	psaA-ycf3	5	TTTTTGAGAAATGCCCCGGT	CAGAGGCTTGGTTCGATCAA	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.388325	45148	45845	909-914	1.000000	78.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49631	50351	771-790	1.000000	81.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49631	50351	779-798	1.000000	81.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	49631	50351	803-822	1.000000	80.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	49631	50351	811-830	1.000000	80.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	49631	50351	794-813	1.000000	79.9
petA-psbJ_p1	petA-psbJ	1	CCCATTACGTGTCCAAGGTC	TTCCGGATTAGGCTCATCCC	20	20	55.0	55.0	58.3	58.9	0.0	19.5	1.209178	67243	68252	1153-1159	1.000000	71.9
petA-psbJ_p2	petA-psbJ	2	GACCCATTACGTGTCCAAGG	TTCCGGATTAGGCTCATCCC	20	20	55.0	55.0	58.3	58.9	0.0	19.5	1.209178	67243	68252	1155-1161	1.000000	71.9
petA-psbJ_p3	petA-psbJ	3	CCCATTACGTGTCCAAGGTC	CGGATTAGGCTCATCCCTGT	20	20	55.0	55.0	58.3	58.9	0.0	19.5	1.214025	67243	68252	1150-1156	1.000000	71.9
petA-psbJ_p4	petA-psbJ	4	GACCCATTACGTGTCCAAGG	CGGATTAGGCTCATCCCTGT	20	20	55.0	55.0	58.3	58.9	0.0	19.5	1.214025	67243	68252	1152-1158	1.000000	71.9
petA-psbJ_p5	petA-psbJ	5	ACGTGTCCAAGGTCTTTTGT	TTCCGGATTAGGCTCATCCC	20	20	45.0	55.0	57.6	58.9	0.0	19.5	1.356650	67243	68252	1147-1153	1.000000	70.7
petG-trnW-CCA_p1	petG-trnW-CCA	1	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.243803	70604	70710	175-175	1.000000	79.9
petG-trnW-CCA_p2	petG-trnW-CCA	2	ACAGACGTGGTGATCAGTTG	TTCAAATCCTACAGGGCGTG	20	20	50.0	50.0	57.8	57.9	0.0	5.5	0.262804	70604	70710	156-156	1.000000	79.9
petG-trnW-CCA_p3	petG-trnW-CCA	3	ACAGACGTGGTGATCAGTTG	GTTCAAATCCTACAGGGCGT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.264278	70604	70710	157-157	1.000000	79.9
petG-trnW-CCA_p4	petG-trnW-CCA	4	GACGTGGTGATCAGTTGGAT	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.6	57.9	0.0	1.4	0.475610	70604	70710	172-172	1.000000	78.1
petG-trnW-CCA_p5	petG-trnW-CCA	5	GACGTGGTGATCAGTTGGAT	TTCAAATCCTACAGGGCGTG	20	20	50.0	50.0	57.6	57.9	0.0	5.5	0.494611	70604	70710	153-153	1.000000	78.1
ndhF-rpl32_p1	ndhF-rpl32	1	CATTCCCACCAGTTCCCAAA	CCCGTGGAAATTGATTTGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016994	116653	117790	1723-1728	1.000000	82.6
ndhF-rpl32_p2	ndhF-rpl32	2	AATACGAGCACATTCCCACC	CCCGTGGAAATTGATTTGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047139	116653	117790	1733-1738	1.000000	82.3
ndhF-rpl32_p3	ndhF-rpl32	3	CGGTCGTGTGAACCAAAAAC	CCCGTGGAAATTGATTTGGC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.191143	116653	117790	1762-1767	1.000000	81.1
ndhF-rpl32_p4	ndhF-rpl32	4	GGTCGTGTGAACCAAAAACC	CCCGTGGAAATTGATTTGGC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.206544	116653	117790	1761-1766	1.000000	81.0
ndhF-rpl32_p5	ndhF-rpl32	5	TTGTGCGGATTTTGCGATTG	CCCGTGGAAATTGATTTGGC	20	20	45.0	50.0	58.3	58.0	0.0	0.0	0.312939	116653	117790	1984-1989	1.000000	80.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCCAAATCAATTTCCACGGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.133029	117953	118649	747-796	1.000000	85.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCCAAATCAATTTCCACGGG	ACGTTTCGGTTGAAAGACCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.035755	117953	118649	1265-1321	1.000000	85.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCCAAATCAATTTCCACGGG	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.205047	117953	118649	746-795	1.000000	84.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCCAAATCAATTTCCACGGG	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.205047	117953	118649	745-794	1.000000	84.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCCAAATCAATTTCCACGGG	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.193756	117953	118649	789-789	0.333333	51.8
ccsA_p1	ccsA	1	ATTTCAAACAAGCCGCCATG	TGGACCACGCGAACTTTTTA	20	20	45.0	45.0	57.9	58.0	0.0	15.9	0.132338	118838	119803	1520-1534	1.000000	80.7
ccsA_p2	ccsA	2	TAGACACGCTGCTCTTAGGA	TGGACCACGCGAACTTTTTA	20	20	50.0	45.0	57.9	58.0	0.0	15.9	0.173449	118838	119803	1490-1504	1.000000	80.4
ccsA_p3	ccsA	3	AAATTGGTAGACACGCTGCT	TGGACCACGCGAACTTTTTA	20	20	45.0	45.0	57.8	58.0	0.0	15.9	0.234176	118838	119803	1497-1511	1.000000	79.9
ccsA_p4	ccsA	4	GGTAGACACGCTGCTCTTAG	TGGACCACGCGAACTTTTTA	20	20	55.0	45.0	57.8	58.0	0.0	15.9	0.245467	118838	119803	1492-1506	1.000000	79.8
ccsA_p5	ccsA	5	GTAGACACGCTGCTCTTAGG	TGGACCACGCGAACTTTTTA	20	20	55.0	45.0	57.8	58.0	0.0	15.9	0.245467	118838	119803	1491-1505	1.000000	79.8
ccsA-ndhD_p1	ccsA-ndhD	1	CGGCAATTGTAGCTTCTATGG	TGGTTCTCTCGCTATCAGTTG	21	21	47.6	47.6	57.1	57.5	7.4	0.0	3.395871	119804	120036	404-411	1.000000	56.2
ccsA-ndhD_p2	ccsA-ndhD	2	CGGCAATTGTAGCTTCTATGG	GGTTCTCTCGCTATCAGTTGA	21	21	47.6	47.6	57.1	57.2	7.4	0.0	3.670185	119804	120036	403-410	1.000000	54.0
ccsA-ndhD_p3	ccsA-ndhD	3	GGCAATTGTAGCTTCTATGGGA	TGGTTCTCTCGCTATCAGTTG	22	21	45.5	47.6	58.2	57.5	7.4	0.0	3.689831	119804	120036	403-410	1.000000	53.8
ccsA-ndhD_p4	ccsA-ndhD	4	GGCAATTGTAGCTTCTATGGG	TGGTTCTCTCGCTATCAGTTG	21	21	47.6	47.6	56.7	57.5	7.4	0.0	3.782301	119804	120036	403-410	1.000000	53.1
ccsA-ndhD_p5	ccsA-ndhD	5	TGAATTCGGCAATTGTAGCT	TGGTTCTCTCGCTATCAGTTG	20	21	40.0	47.6	55.7	57.5	7.4	0.0	3.782453	119804	120036	410-417	1.000000	53.0
ycf1_p1	ycf1	1	TCAATTTTGGTGGACGAGGT	ACAATTTTGTTGCCAGTAAACCA	20	23	45.0	34.8	57.4	57.9	0.0	0.0	3.740793	132734	132853	216-216	1.000000	53.0
ycf1_p2	ycf1	2	GTCAATTTTGGTGGACGAGG	ACAATTTTGTTGCCAGTAAACCA	20	23	50.0	34.8	57.0	57.9	0.0	0.0	4.080684	132734	132853	217-217	1.000000	50.3
ycf1_p3	ycf1	3	CCTTGTCAATTTTGGTGGACG	ACAATTTTGTTGCCAGTAAACCA	21	23	47.6	34.8	58.0	57.9	0.0	0.0	4.103211	132734	132853	221-221	1.000000	50.1
ycf1_p4	ycf1	4	TGGTGGACGAGGTACTTTTT	ACAATTTTGTTGCCAGTAAACCA	20	23	45.0	34.8	56.7	57.9	0.0	0.0	4.398082	132734	132853	209-209	1.000000	47.9
ycf1_p5	ycf1	5	TCAATTTTGGTGGACGAGGT	CAATTTTGTTGCCAGTAAACCA	20	22	45.0	36.4	57.4	56.3	0.0	0.0	4.397994	132734	132853	215-215	1.000000	47.8