Hotspot

psbA

LSC · 316-1377 · 1062 bp

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Pi 0.0022
MarkerSeek score 33.30
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
9
pi
0.0022
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 GATCCACTTGGCTACATCCG AATGAAAACTGCAAGCACGG 55.0 45.0 58.1 57.9 1663-1713 bp 1.0 83.2
psbA_p2 CAATCCACTGCCTTGATCCA AATGAAAACTGCAAGCACGG 50.0 45.0 57.9 57.9 1677-1727 bp 1.0 83.1
psbA_p3 AATCCACTGCCTTGATCCAC AATGAAAACTGCAAGCACGG 50.0 45.0 57.9 57.9 1676-1726 bp 1.0 83.1
psbA_p4 CCAACCCGCTCAATAGAACA AATGAAAACTGCAAGCACGG 50.0 45.0 57.9 57.9 1488-1513 bp 1.0 79.7
psbA_p5 ACCAACCCGCTCAATAGAAC AATGAAAACTGCAAGCACGG 50.0 45.0 57.9 57.9 1489-1514 bp 1.0 79.7