Hotspot

clpP1

LSC · 70656-72920 · 2265 bp

Pi 0.0244

MarkerSeek score 67.20

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2265
variable_sites: 166
pi: 0.0244
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	CTAGTACATGTTCCTCGGCG	GCAAATGCCGCCATATTTGT	55.0	45.0	57.9	58.1	2725-2796 bp	0.888889	80.3
clpP1_p2	AGTCGCACATACACCCTAGT	GCAAATGCCGCCATATTTGT	50.0	45.0	58.2	58.1	2740-2811 bp	0.888889	80.0
clpP1_p3	AGTGACCAGAGCTCTTCCAT	GCAAATGCCGCCATATTTGT	50.0	45.0	58.1	58.1	2833-2885 bp	0.777778	71.4
clpP1_p4	AGTGACCAGAGCTCTTCCAT	GCAATGGGGGTTGATACCTT	50.0	50.0	58.1	57.8	2862-2914 bp	0.777778	70.6
clpP1_p5	AGTGACCAGAGCTCTTCCAT	CCCTGTTCAGTGGATTGGAG	50.0	55.0	58.1	57.9	2984 bp	0.111111	43.1