Hotspot

rpl32-trnL-UAG

SSC · 118785-119262 · 478 bp

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Pi 0.0446
MarkerSeek score 81.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
478
variable_sites
63
pi
0.0446
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGACATTCGGCAGCATTA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 566-644 bp 1.0 88.6
rpl32-trnL-UAG_p2 AGGACATTCGGCAGCATTAA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 565-643 bp 1.0 88.6
rpl32-trnL-UAG_p3 AAGGACATTCGGCAGCATTA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 567-645 bp 1.0 88.6
rpl32-trnL-UAG_p4 AGGACATTCGGCAGCATTAA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 566-644 bp 1.0 88.6
rpl32-trnL-UAG_p5 AAAGGACATTCGGCAGCATT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.5 58.0 567-645 bp 1.0 88.3