Hotspot

psbE-petL

LSC · 64842-66085 · 1244 bp

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Pi 0.022
MarkerSeek score 68.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1244
variable_sites
85
pi
0.022
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1283-1330 bp 1.0 74.3
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1278-1325 bp 1.0 73.9
psbE-petL_p3 CGCAAAAGAACGTTCTCCTG AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.7 56.3 1289-1336 bp 1.0 73.1
psbE-petL_p4 TCTCCTGTGCTTCCAGACAT AGCTGCTAGTAGAAAACCGA 50.0 45.0 58.4 56.3 1276-1323 bp 1.0 72.7
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGAA 50.0 42.9 57.8 57.0 1283-1330 bp 1.0 72.3