Hotspot

rps4-trnT-UGU

LSC · 46340-46964 · 625 bp

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Pi 0.0414
MarkerSeek score 83.20
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
625
variable_sites
89
pi
0.0414
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 GAAACGAGGCCCTCTGTAAC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.3 57.9 716-750 bp 1.0 88.7
rps4-trnT-UGU_p2 GAAACGAGGCCCTCTGTAAC GATGGTCATCGGTTCGACTC 55.0 55.0 58.3 58.2 685-719 bp 1.0 88.5
rps4-trnT-UGU_p3 TTTTTGAAACGAGGCCCTCT CTCAGAGGTTAGAGCATCGC 45.0 55.0 57.6 57.9 721-755 bp 1.0 88.1
rps4-trnT-UGU_p4 TTTTTGAAACGAGGCCCTCT GATGGTCATCGGTTCGACTC 45.0 55.0 57.6 58.2 690-724 bp 1.0 87.9
rps4-trnT-UGU_p5 TTTTGAAACGAGGCCCTCTG CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.4 57.9 720-754 bp 1.0 87.8