Hotspot

psaA-ycf3

LSC · 41646-42434 · 789 bp

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Pi 0.0326
MarkerSeek score 83.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
789
variable_sites
82
pi
0.0326
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 AGGTTTTTACGGGGTCCCTA TTGAAGATCACGAGGCGTTT 50.0 45.0 58.0 57.8 850-1040 bp 1.0 89.6
psaA-ycf3_p2 AGGTTTTTACGGGGTCCCTA TTGGTTGAAGATCACGAGGC 50.0 50.0 58.0 58.2 854-1044 bp 1.0 89.3
psaA-ycf3_p3 CTGGCCCATTCCTCAAATGA TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 869-1059 bp 1.0 88.6
psaA-ycf3_p4 TCTGGCCCATTCCTCAAATG TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 870-1060 bp 1.0 88.6
psaA-ycf3_p5 TGGCCCATTCCTCAAATGAG TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 868-1058 bp 1.0 88.6