Hotspot

psbC-trnS-UGA

LSC · 35108-35397 · 290 bp

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Pi 0.0498
MarkerSeek score 81.10
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
290
variable_sites
39
pi
0.0498
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCGATGACCCC GGTTTATAGCCCCGGTCTTG 55.0 55.0 58.3 58.0 390-428 bp 1.0 88.2
psbC-trnS-UGA_p2 CCTGTTCTTTCGATGACCCC GCTGAGTGGTTTATAGCCCC 55.0 55.0 58.3 58.0 397-435 bp 1.0 88.1
psbC-trnS-UGA_p3 CCTGTTCTTTCGATGACCCC GGCTGAGTGGTTTATAGCCC 55.0 55.0 58.3 58.0 398-436 bp 1.0 88.1
psbC-trnS-UGA_p4 CCTGTTCTTTCGATGACCCC GTTGGAGAGATGGCTGAGTG 55.0 55.0 58.3 58.0 409-447 bp 1.0 87.9
psbC-trnS-UGA_p5 CCTGTTCTTTCGATGACCCC ATAGCCCCGGTCTTGAAAAC 55.0 50.0 58.3 57.9 385-423 bp 1.0 87.7