Hotspot

petN-psbM

LSC · 28122-29091 · 970 bp

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Pi 0.0282
MarkerSeek score 68.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
970
variable_sites
79
pi
0.0282
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CACCGAAATATTCCCCAGCA TGCGAAGTAAAAACCACGGA 50.0 45.0 57.9 58.0 1711-1759 bp 1.0 89.5
petN-psbM_p2 CACCGAAATATTCCCCAGCA ACCCCGAAAAAGACGTTTCA 50.0 45.0 57.9 58.0 2031-2083 bp 1.0 89.3
petN-psbM_p3 CCCTTTCACTCGTCGTATGG ACCCCGAAAAAGACGTTTCA 55.0 45.0 58.1 58.0 1451-1540 bp 1.0 87.8
petN-psbM_p4 CCTTTCACTCGTCGTATGGG ACCCCGAAAAAGACGTTTCA 55.0 45.0 58.1 58.0 1450-1539 bp 1.0 87.8
petN-psbM_p5 CTTTCACTCGTCGTATGGGG ACCCCGAAAAAGACGTTTCA 55.0 45.0 58.1 58.0 1449-1538 bp 1.0 87.8