Hotspot

rpoB-trnC-GCA

LSC · 26139-27373 · 1235 bp

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Pi 0.0238
MarkerSeek score 66.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1235
variable_sites
94
pi
0.0238
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TTCCCTCATTTCCATCCCGG TTTGTTGACTAGGCGACACC 55.0 50.0 59.4 58.1 875-1417 bp 1.0 77.2
rpoB-trnC-GCA_p2 TTCCCTCATTTCCATCCCGG AAAGGATTTGCAGTCCTCCG 55.0 50.0 59.4 58.2 836-1378 bp 1.0 77.0
rpoB-trnC-GCA_p3 TTCCCTCATTTCCATCCCGG CTAGGCGACACCCAGATTTG 55.0 55.0 59.4 58.3 867-1409 bp 1.0 75.5
rpoB-trnC-GCA_p4 CCCTCATTTCCATCCCGGA TTTGTTGACTAGGCGACACC 57.9 50.0 58.8 58.1 873-1415 bp 1.0 74.7
rpoB-trnC-GCA_p5 TCCCTCATTTCCATCCCGG TTTGTTGACTAGGCGACACC 57.9 50.0 58.8 58.1 874-1416 bp 1.0 74.7