Hotspot

atpH-atpI

LSC · 13277-13917 · 641 bp

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Pi 0.0248
MarkerSeek score 72.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
641
variable_sites
55
pi
0.0248
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TTCAGGCTGTCTCGCAATAC CAAGCGGTATTCAGGCTCTT 50.0 50.0 58.0 58.0 848-914 bp 1.0 88.1
atpH-atpI_p2 CCTCATCCTCAAATCAGCCC CAAGCGGTATTCAGGCTCTT 55.0 50.0 58.0 58.0 1089-1162 bp 1.0 87.8
atpH-atpI_p3 TACCTTCTACAGCTTGGCCT CAAGCGGTATTCAGGCTCTT 50.0 50.0 58.0 58.0 831-897 bp 1.0 87.8
atpH-atpI_p4 TTCAGGCTGTCTCGCAATAC GAATTAGCCGCACCTACGAA 50.0 50.0 58.0 58.1 1140-1206 bp 1.0 85.7
atpH-atpI_p5 TTCAGGCTGTCTCGCAATAC GCCGCACCTACGAATGATAT 50.0 50.0 58.0 57.9 1134-1200 bp 1.0 85.6