Hotspot

psbI-trnS-GCU

LSC · 7678-7781 · 104 bp

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Pi 0.0364
MarkerSeek score 72.80
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
104
variable_sites
13
pi
0.0364
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA GTCATTCGTACCGAGGGTTC 50.0 55.0 57.1 58.1 154-170 bp 1.0 82.2
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA TAGGGAGAGATGGCTGAGTG 50.0 55.0 57.1 57.9 208-224 bp 1.0 80.7
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGAA GTCATTCGTACCGAGGGTTC 47.6 55.0 57.8 58.1 154-170 bp 1.0 79.9
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGA AGTCATTCGTACCGAGGGTT 50.0 50.0 57.1 58.4 155-171 bp 1.0 79.3
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGAA TAGGGAGAGATGGCTGAGTG 47.6 55.0 57.8 57.9 208-224 bp 1.0 78.3