Hotspot

rps16-trnQ-UUG

LSC · 6053-6567 · 515 bp

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Pi 0.0745
MarkerSeek score 76.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
515
variable_sites
120
pi
0.0745
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCGTTGCTTTCTACCACATC GTAGAAATCTCAGTGGGGCG 50.0 55.0 57.5 58.1 623-679 bp 1.0 87.3
rps16-trnQ-UUG_p2 GCGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 562-618 bp 1.0 87.2
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG GTAGAAATCTCAGTGGGGCG 50.0 55.0 57.2 58.1 622-678 bp 1.0 85.2
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 561-617 bp 1.0 85.1
rps16-trnQ-UUG_p5 GCGTTGCTTTCTACCACATC TAGAAATCTCAGTGGGGCGT 50.0 50.0 57.5 58.4 622-678 bp 1.0 84.3