Hotspot

trnK-UUU-rps16

LSC · 4198-4919 · 722 bp

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Pi 0.0252
MarkerSeek score 71.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
722
variable_sites
60
pi
0.0252
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGAACTTCGCCCTAATGAAACA 50.0 40.9 58.2 58.0 787-814 bp 0.777778 54.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGAACTTCGCCCTAATGAAACA 50.0 40.9 58.2 58.0 786-813 bp 0.777778 54.7
trnK-UUU-rps16_p3 AAAAGAACCTAGCCGCACTT AGAACTTCGCCCTAATGAAACA 45.0 40.9 57.7 58.0 808-835 bp 0.777778 53.9
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AGAACTTCGCCCTAATGAAACA 50.0 40.9 58.3 58.0 796-823 bp 0.777778 53.8
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC AGAACTTCGCCCTAATGAAACA 50.0 40.9 57.5 58.0 773-800 bp 0.777778 52.4