Hotspot

rps2

LSC · 15508-16218 · 711 bp

Back to Ruellia

Pi 0.00281

MarkerSeek score 47.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACGTGAGTAAAGCCAAAGCA	GGGTCGAATCGATTGGTTGA	45.0	50.0	58.0	58.0	1831-1834 bp	1.0	80.0
rps2_p2	CAAGGGCCGTATTCTTCTCC	GGGTCGAATCGATTGGTTGA	55.0	50.0	58.0	58.0	1679-1682 bp	1.0	80.0
rps2_p3	CCAAGGGCCGTATTCTTCTC	GGGTCGAATCGATTGGTTGA	55.0	50.0	58.0	58.0	1680-1683 bp	1.0	80.0
rps2_p4	GGAAACCCCCTATTTGCCAA	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.1	58.0	1498-1501 bp	1.0	79.9
rps2_p5	TTGGAAACCCCCTATTTGCC	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.1	58.0	1500-1503 bp	1.0	79.9