Hotspot

rps16

LSC · 4678-5815 · 1138 bp

Back to Ruellia

Pi 0.00703

MarkerSeek score 57.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1138
variable_sites: 8
pi: 0.00703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAAGAATACAGCGGAGGGG	CCGGTTCCAAATTCGAGGAA	50.0	50.0	57.6	58.2	1254-1256 bp	1.0	75.7
rps16_p2	AAAAGAATACAGCGGAGGGG	ATGGACAACCGAAGCAATGA	50.0	45.0	57.6	57.8	1308-1310 bp	1.0	75.5
rps16_p3	AAAAGAATACAGCGGAGGGG	GTCTATGGACAACCGAAGCA	50.0	50.0	57.6	57.6	1312-1314 bp	1.0	74.1
rps16_p4	AAAAGAATACAGCGGAGGGG	TACCGGTTCCAAATTCGAGG	50.0	50.0	57.6	57.6	1256-1258 bp	1.0	74.0
rps16_p5	AAAAGAATACAGCGGAGGGG	GGACAACCGAAGCAATGACT	50.0	50.0	57.6	58.5	1306-1308 bp	1.0	73.3