Hotspot

rpl16

LSC · 80607-81855 · 1249 bp

Back to Ruellia

Pi 0.00801

MarkerSeek score 48.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1249
variable_sites: 10
pi: 0.00801
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCCAAAACTCGTTTCTGTTGG	CCGTCTATGCATAGATTGAAGA	47.6	40.9	57.9	55.5	1400-1401 bp	1.0	40.6
rpl16_p2	CCCAAAACTCGTTTCTGTTGG	CCGTCTATGCATAGATTGAAGAA	47.6	39.1	57.9	56.2	1400-1401 bp	1.0	40.6
rpl16_p3	CCCAAAACTCGTTTCTGTTGG	CCGTCTATGCATAGATTGAAG	47.6	42.9	57.9	54.0	1400-1401 bp	1.0	40.6
rpl16_p4	CCCAAAACTCGTTTCTGTTGG	CCGTCTATGCATAGATTGAAGAAA	47.6	37.5	57.9	56.9	1400-1401 bp	1.0	40.6
rpl16_p5	CCAAAACTCGTTTCTGTTGGT	CCGTCTATGCATAGATTGAAGA	42.9	40.9	57.2	55.5	1399-1400 bp	1.0	40.6