Hotspot

ndhD

SSC · 120282-121736 · 1455 bp

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Pi 0.00275

MarkerSeek score 45.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1455
variable_sites: 4
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GATACCCTGGGACGGATGTA	AGCGGTTCTTACGGATAGGA	55.0	50.0	58.0	57.9	2038 bp	1.0	79.9
ndhD_p2	GATACCCTGGGACGGATGTA	TTGGAGTTGGGACCCAAAAG	55.0	50.0	58.0	57.9	2260 bp	1.0	79.7
ndhD_p3	GATACCCTGGGACGGATGTA	CTAACGAAGCATGGGGATCC	55.0	55.0	58.0	58.1	2282 bp	1.0	79.5
ndhD_p4	AACAAATCGCTTCTGCTCCA	AGCGGTTCTTACGGATAGGA	45.0	50.0	58.1	57.9	2012 bp	1.0	79.1
ndhD_p5	ACAAATCGCTTCTGCTCCAA	AGCGGTTCTTACGGATAGGA	45.0	50.0	58.1	57.9	2011 bp	1.0	79.1