Hotspot

trnF-GAA-ndhJ

LSC · 48220-48916 · 697 bp

Back to Ruellia

Pi 0.00862

MarkerSeek score 63.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 6
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGCTAGGATGGC	55.0	50.0	58.0	57.9	842-843 bp	1.0	79.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGCTAGGATGGC	50.0	50.0	58.1	57.9	806-807 bp	1.0	79.2
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TGAAAGTTGGCTAGGATGGC	55.0	50.0	57.9	57.9	830-831 bp	1.0	78.6
trnF-GAA-ndhJ_p4	ATGAGGGTGGGATGCTACAT	TGAAAGTTGGCTAGGATGGC	50.0	50.0	58.2	57.9	873-874 bp	1.0	78.2
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCTGAAAGTTGGCTAGGAT	55.0	50.0	58.0	57.6	845-846 bp	1.0	77.2