Hotspot

rps4-trnT-UGU

LSC · 46181-46669 · 489 bp

Back to Ruellia

Pi 0.00442

MarkerSeek score 63.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 2
pi: 0.00442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	ATCGCTTCCAGCTTTAGTCC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	609-645 bp	1.0	80.7
rps4-trnT-UGU_p2	AGTCCTGGTAAAGTCCCCAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	578-614 bp	1.0	80.5
rps4-trnT-UGU_p3	AGCGCGATTGGTTTCTAAGA	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	628-664 bp	1.0	80.1
rps4-trnT-UGU_p4	ATCGCTTCCAGCTTTAGTCC	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.7	57.8	577-613 bp	1.0	79.8
rps4-trnT-UGU_p5	AGTCCTGGTAAAGTCCCCAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	546-582 bp	1.0	79.6