Hotspot

trnE-UUC-trnT-GGU

LSC · 30950-31564 · 615 bp

Back to Ruellia

Pi 0.00492
MarkerSeek score 62.40
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
615
variable_sites
3
pi
0.00492
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 751-756 bp 1.0 79.5
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 714-719 bp 1.0 79.5
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 730-735 bp 1.0 79.1
trnE-UUC-trnT-GGU_p4 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 693-698 bp 1.0 79.0
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 727-732 bp 1.0 78.9