Hotspot

trnC-GCA-petN

LSC · 28052-28895 · 844 bp

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Pi 0.00732
MarkerSeek score 65.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
844
variable_sites
6
pi
0.00732
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 935-954 bp 1.0 81.8
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 933-952 bp 1.0 81.8
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 936-955 bp 1.0 81.2
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 881-900 bp 1.0 80.8
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT TTCTTCCCCACACTACGAGT 50.0 50.0 58.4 58.0 951-970 bp 1.0 79.6