Hotspot

atpH-atpI

LSC · 13523-14540 · 1018 bp

Back to Ruellia

Pi 0.00596

MarkerSeek score 53.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1018
variable_sites: 6
pi: 0.00596
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1142-1152 bp	1.0	78.3
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCT	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.6	58.3	1182-1192 bp	1.0	75.7
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.1	57.4	1067-1077 bp	1.0	75.7
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	AGTGGTTCCTATCCCTGTCA	45.0	50.0	58.1	57.4	1153-1163 bp	1.0	75.2
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	TCAGTGGTTCCTATCCCTGT	45.0	50.0	58.1	57.4	1155-1165 bp	1.0	75.2