Hotspot

ndhA

SSC · 121540-123881 · 2342 bp

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Pi 0.0144
MarkerSeek score 67.20
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2342
variable_sites
341
pi
0.0144
unique_haplotype_count
72
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TCTTGGTTGAGACAAGTTGTGA TGGGAGAAGTTGATCGTTGA 40.9 45.0 57.8 56.5 2330-2436 bp 0.976471 61.2
ndhA_p2 TCTCTTGGTTGAGACAAGTTGT TGGGAGAAGTTGATCGTTGA 40.9 45.0 57.5 56.5 2332-2438 bp 0.988235 59.5
ndhA_p3 TCTTGGTTGAGACAAGTTGTGA TGGGAGAAGTTGATCGTTGAA 40.9 42.9 57.8 57.2 2330-2436 bp 0.976471 59.2
ndhA_p4 CTCTTGGTTGAGACAAGTTGTG TGGGAGAAGTTGATCGTTGA 45.5 45.0 57.5 56.5 2331-2437 bp 0.976471 58.6
ndhA_p5 CTTGGTTGAGACAAGTTGTGA TGGGAGAAGTTGATCGTTGA 42.9 45.0 56.3 56.5 2329-2435 bp 0.976471 57.5